cm 3321 - biochemistry lab (1) sp

the biochemistry laboratory course includes the determination of protein concentration, enzyme kinetics, sds-polyacrylamide gel electrophoresis, isoelectric focusing and protein isolation by high speed centrifugation and various chromatographic techniques. additionally, the manipulation of dna including the transformation of e.coli with a plasmid containing a foreign gene, expression of that gene, isolation of the plasmid from the bacteria and restriction analysis of the plasmid on agarose gels.